Journal: Biomedicines
Article Title: In Vitro Screening Studies on Eight Commercial Essential Oils-Derived Compounds to Identify Promising Natural Agents for the Prevention of Osteoporosis
doi: 10.3390/biomedicines11041095
Figure Lengend Snippet: Influence of EO-derived compounds on mouse primary calvarial preosteoblast (MC3T3-E1 Subclone 4) proliferation in vitro. The total cell number was calculated using the Lactate Dehydrogenase Activity (LDH) Assay Kit (DT, doubling time in hours; * statistically significant results between indicated groups, n = 3, p < 0.05, one-way ANOVA followed by Tukey’s test).
Article Snippet: The screening cytotoxicity test, cell proliferation assay, and osteogenic differentiation tests were carried out using a mouse primary calvarial preosteoblast cell line (MC3T3-E1 Subclone 4, CRL-2593, ATCC-LGC standards, Teddington, UK).
Techniques: Derivative Assay, In Vitro, Activity Assay, Lactate Dehydrogenase Assay